Apoptosis Methods and Protocols, 2nd Edition

Price: $189.00
Catalog No.: B60007
Publisher/Vendor: Humana Press
Author: Peter Erhardt and Ambrus Toth (Editors)
Pages: 360
Format: Hardcover Book
Apoptosis Methods and Protocols, 2nd Edition

The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Apoptosis: Methods and Protocols, Second Edition is a technical reference about significant methodologies used in the field, and offers beginners and experienced researchers tools to illuminate the phenomena of programmed cell death.

Key Features

  • Presents methods used to investigate the critical steps in apoptotic cell death
  • Written by leading researchers in the field
  • Details the process of how to induce, measure and differentiate apoptosis in various models
  • Covers a wide range of model systems for apoptosis detection from cell-based assays to measurements at the level of organ or organism
  • Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, approaches to data interpretation, and tips for troubleshooting
  • Serves as a comprehensive guide to methodologies applied to elucidate the molecular mechanisms of apoptosis

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